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KMID : 0352720090330030199
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Journal of Ginseng Research
2009 Volume.33 No. 3 p.199 ~ p.205
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Genetic Polymorphism of Microsatellite Markers in Panax ginseng C.A. Meyer
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Park Sun-Wha
Chung Ki-Wha
Hyun Young-Se
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Abstract
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Ginseng (Panax ginseng C.A. Meyer) is one of the most important medicinal plants in East Asia. Microsatellite or simple sequence repeat (SSR) markers are used in obtaining genetic analysis and authentication in many plants. The present study examined five microsatellites in conjunction with P. ginseng in Korea. The total observed allelic number was 17 (mean = 3.4), and gene diversities varied from 0.078 to 0.543 with an average of 0.314. Through a combined analysis of five loci in 100 ginseng samples, 44 different combined genotypes were observed. Expected and observed heterozygosites ranged from 0.077 to 0.541 (mean = 0.313) and 0.040 to 0.130 (0.083), respectively. All examined loci exhibited deficiency of heterozygosity and deviation from the Hardy-Weinberg equilibrium. Such results may be explained by the non-random mating and inbreeding that has occurred for several hundred years. These microsatellite markers could be used for the study of molecular genetics and the establishment of DNA marker database, as well as authentication of ginseng species and chromosomal mapping of QTL loci in P. ginseng.
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KEYWORD
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Panax ginseng, microsatellite, simple sequence repeats (SSR), polymorphism
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